Pooja from Germany applies for Blue Card Germany

Biologists, botanists, zoologists and related professionals

Personal data:

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with the following Applicants-ID: 16389


Name of profession: Biologists, botanists, zoologists and related professionals Name of latest business sector: Research Technician Latest occupation/title: Laboratory Officer Latest main activities: Molecular and cell culture techniques.


Completed education: master Title or Qualification: Master of Science Specialisation: Microbiology Additional trainings: Molecular and cell culture laboratory techniques at ACTREC,Mumbai and ICSCCB,Pune (India). ZIBI summer school at Humboldt University,Germany.

Preferred countries:

1. Preferred country | region / city: Germany | Frankfurt,Berlin,Munich 2. Preferred country | region / city: Netherlands | 3. Preferred country | region / city: Sweden |

Language skills:

1. Language skills: english => excellent 2. Language skills: german => reasonable 3. Language skills: Marathi, Hindi => excellent

Personal note /detailed application:


• Abgeschlossenes Studium: Master of Science : Fach- Mikrobiologie (2 Jahrgänge ) am Abasaheb Garware college, Pune und an der Universität Pune, Indien)
Zeitraum: 06/2008 - 04/2010
Ort: Universität Pune, (Indian)
Abschlussqualifikation: Master-Abschluss (Indien)
Abschlussnote: ausreichend
• Thema der Abschlussarbeit: „Enhancement of Alcohol Production using Invertase Producer“.
( „Verbesserung der Alkoholproduktion mit Invertase- Produzenten“).
Note Abschlussarbeit: A (Sehr gut).

• Abgeschlossenes Studium: Bachelor of Science : Fach- Industriell Mikrobiologie (3 Jahrgänge) am Abasaheb Garware college, Pune und an der Universität Pune, Indien)
Zeitraum: 06/2005 - 04/2008
Ort: Universität Pune, (Indian)
Note: Gut.

• Abschluss der Abitur :
Zeitraum: 06/2003 - 04/2005
Ort: N.M.V. Girls Highschool and Junior College (Indien)
Note:: Sehr gut

• „Enhancement of Alcohol production using Invertase Producers“
(,, Verbesserung der Alkoholproduktion mit Invertase-Produzenten ‘‘)

Zeitraum: 08/2009 – 01/2010 (6 Monate)
Ort: Universität Pune, (Indian)
Tätigkeit: Master of science
Projektbeschreibung: To find the yield of alcohol with different matrices ( eg baby corn ) & use
sucrose as a substrate for ethanol fermentation.

? Screening , Osmotolerence , invertase activity , fermentation and distillation.
? For immobilization, baby corn , wood chips and corn grains matrices were used.
? The fermentation was carried out with the static condition and finally alcohol obtained by the
distillation method and estimated by Cerric ammonium reagent.

• Deutschkurs: (Grund Deutsch, Goethe Institute e.V.)
A1- 20.02.2013 : 86%
A2 - 22.04.2013 : 60%
B1 – Currently Learning

Deutsch: Ausreichend
Englisch: Gut
Marathi : Muttersprache

Technische Fähigkeiten:-
• Optimization of PCR condition and Troubleshooting for PCR. Enzymatic Treatment of PCR product for Sanger Sequencing , DNA cycle sequencing reaction, DNA cycle sequencing post PCR clean up and Capillary electrophoresis of cleaned products. Interpretation on Chromatogram for frameshift mutation in BRCA 1 gene.

• RNA extraction, cDNA synthesis, Reverse Transcriptase PCR (RT – PCR), Gel elution of aberrant PCR product for sequencing.

• TaqMan Assay. Interpretation by amplification plot and Multicomponent plot, allelic discrimination plot.

• Confirmation Sensitive Gel Electrophoresis and Agarose Gel Electrophoresis of Restriction digestion.

• MLPA for Large Genomic Rearrangements. Interpretation using Coffalyser software.

• Immunodiffusion for tetanus and diphtheria antigen , preparation of O & H - antigen at National Toxicology Center . Agglutination reaction for titer determination , basic cell culture of chicken embryos , animal vaccination technique through different routes .

• Basic cell culture techniques; media preparation, genomic DNA isolation, estimation and agarose gel electrophoresis. RNA extraction, cDNA preparation, PCR and agarose gel electrophoresis. Protein extraction, estimation by SDS PAGE and coomassie staining of proteins. Freezing and thawing of cells and MTT assay for proliferation/ cytotoxicity.


1. Name des Arbeitgebers:
Chaitanya Hospital and Stem Cell Center
(July 2013- September 2014)

Adresse der Arbeitgeber:
107 Yamunashree society, Nawashya Maruti
Chowk , Pune 411030
Position: Junior Laboratory Officer
Wichtigste Tätigkeiten und Aufgaben :

Isolation, cultivation and maintenance of Hematopoietic Stem Cells and Mono Nuclear Cells from the bone marrow. Cultivation and maintenance of Human umbilical cord tissue derived Wharton’s Jelly Mesenchymal Stem Cells (MSC). Expansion of isolated MSC and Cryopreservation. Platelet Rich Plasma (PRP) Isolation. To develop Standard Operating Procedure. To supervise entire Lab Documentation. To help in therapeutic application of isolated stem cells in patients.

2. Name der Arbeitgeber:
TechKnit (IT Enabled Services ) Pvt. Limited.
( October 2010 – January 2013 ) and (September 2013 to July 2015)
Adresse des Arbeitgebers:
Sneh-Leela Chambers, 1237 Apte Road, Deccan Gymkhana, Pune. (India) PIN-411004.

Position: E-Tutor
Wichtigste Tätigkeiten und Aufgaben :

To engage& encourage students in active learning as well as enhance their motivation , rather than simply " giving answers " or doing " homework " for them in a professional environment . Topics for teaching are especially " General Biology " & "Introduction to Human Anatomy & Physiology .

Konferenzen und Sommer Schule:-
• 7 Annual Conference of the International Association of Neurorestoratology.1st Annual Meeting of the Society for Stem Cell India participated . 11 Annual Conference of the Global College of Neuroprotection and regeneration. 2 Annual Meeting of the Indian Federation of Neurorehabilitation . ( Mumabai - India)
• 2nd Indian Cancer Genetics Conference and Workshop at Advanced Center for Treatment and Education in Center (ACTREC), Tata Memorial Center (TMC). (Mumbai- India).
• Poster presentation zum Thema :" Clinical trial opened the gate for patients with Cerebral Palsy with the use of a combination of autologous bone marrow-derived mononuclear cells ( MNC) ,mesenchymanl stem cells (MSC) and Wharton's jelly derived mesenchymal stem cells ( MSC) " : 4 International Conference on Stem Cells and Cancer (Mumbai - Haffkine Institue ) 3. Preis .
• Folgende Labortechniken in Lab-Sessions während ZIBI Sommerschule , an der Humboldt-Universität in Berlin, Deutschland (2012) : Cell Sorting . Mouse splenic CD4 + T cell isolation & MACS sort , titration of bead concentration and influence on cell purity and yield. b . Multicolor FACS staining of the students PBMCs. c .Cell cycle analysis of intracellular cytokine staining of mouse cells.


• yoga machen
• reisen


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